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. 2013 Jul 2;15(2):160–165.

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Fig 3 — Morphological changes of MDA-MB-231 cells after 24 hours of germacrone (A), curdione (B) and furanodiene (C) alone or in combination treatment (up) and JC-1 staining (down). After A and B treatment, there is no obvious morphological changes in the cells. C treated group showed decreased cell number. However, after combination of these compounds, the cell numbers decreased and the cells became round. Some dead cells were also observed in C, AC, BC and ABC treated groups. The red fluorescence suggested that the mitochondrial membrane potential (Δψm) was high while the green fluorescence suggested that the Δψm was low. The magnification for morphological study and JC-1 staining study were ×20 and ×40 respectively.