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. 2013 Jul 2;15(2):190–197.

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Fig 3 — Phase contrast microscopy of OG2 mouse ESC line treated with Royan-hLIF compared to commercial hLIF as the control group, after 10 passages. A. Commercial hLIF (10 ng/ml). B. Royan-hLIF (10 ng/ml). C. Alkaline phosphatase (ALP) staining of the commercial hLIF group. D. ALP staining of Royan-hLIF. E. Immuonofluorescence staining for OCT4 as a pluripotency marker in the commercial hLIF group and F in the Royan-hLIF group. G. Immuonofluorescence staining for SSEA1 as a pluripotency marker in the commercial hLIF group and H in the Royan-hLIF group. I. Quantification of positive cells by immunoflourescence staining. J. MTT assay after 1, 5 and 10 passages of mouse ESCs in the presence of commercial and Royan hLIF. K. Embryoid body (EB) formation for cardiomyocyte differentiation of mouse ESCs after 10 passages using Royan-hLIF. L. Mouse ESC-derived beating colony 5-10 days after differentiation to cardiomyocytes. Results showed that the group treated with 10 ng/ml of Royan hLIF had similar morphological characteristics to the group treated with 10 ng/ml of commercial hLIF. And also mouse ESCs cultured in Royan hLIF for ten passages were induced toward a cardiac cell lineage. According to the results, mouse ESCs cultured with Royan-hLIF efficiently produced beating cells and there was no significant difference between Royan-hLIF and commercial hLIF.