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. Author manuscript; available in PMC: 2013 Jul 16.
Published in final edited form as: Chembiochem. 2011 Dec 23;13(2):259–270. doi: 10.1002/cbic.201100638

Table 2.

Dissociation constants of type I arginine-equivalent arginine mimetic peptides (AMI).

Kd,
μM		 Error ΔG,
kcal mol−1 		ΔΔGaffinity,
kcal mol−1 		ΔΔGspecificity,b
kcal mol−1
Src Arg 5.3 0.3 −7.2 0 −1.0
Dap-Ac 83 2.8 −5.5 1.63 −0.9
Arg(gGly) 104 8.1 −5.4 1.76 −0.9
Arg(gl-Val) 86 3.1 −5.5 1.65 −0.5
Arg(gd-Val) 75 3.2 −5.6 1.57 −0.7
Arg(gl-Phe) 54 4.8 −5.8 1.37 −0.5
Arg(gd-Phe) 75 4.9 −5.6 1.57 −0.7
Arg(gl-Trp ) 54 3.8 −5.8 1.37 −0.9
Arg(gd-Trp) 51 3.6 −5.8 1.34 −1.1
Grb Arg 29 2 −6.2 0
Dap-Ac 388 75 −4.6 1.53
Arg(gGly) 445 75 −4.6 1.60
Arg(gl-Val) 210 21 −5.0 1.17
Arg(gd-Val) 243 33 −4.9 1.25
Arg(gl-Phe) 117 10 −5.3 0.82
Arg(gd-Phe) 254 17 −4.9 1.28
Arg(gl-Trp) 253 16 −4.9 1.28
Arg(gd-Trp) 333 29 −4.7 1.43
a

ΔΔGaffinity = ΔG (peptide) – ΔG (Arg).

b

ΔΔGspecificity = ΔGSrc – ΔGGrb for a given peptide. All peptides bound poorly to Crk (Kd > 250 μM). All experiments were conducted in PBS at 25 °C.