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. Author manuscript; available in PMC: 2013 Jul 16.
Published in final edited form as: Bone. 2013 Jan 19;53(2):478–486. doi: 10.1016/j.bone.2013.01.020

Figure 3. Effect of polyphosphates on calcium chelation.

Figure 3

(A) Various concentrations of PolyP5 and PolyP65 were added to osteogenic media and ionized calcium content was measured using an ion-sensing calcium electrode. Minimal amounts of calcium are chelated by the polyphosphates. Data are presented as mean values ± SE. (B) MC3T3-E1 osteoblast cultures were treated with 10 μM PolyP5 or PolyP65 with the addition of the indicated concentrations of calcium chloride. After 12 days of treatment, culture plates were analyzed for mineralization by von Kossa staining (black). No effect from the additional calcium is seen for the inhibition of mineralization by the two polyphosphates.