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. Author manuscript; available in PMC: 2013 Jul 16.
Published in final edited form as: Bone. 2013 Jan 19;53(2):478–486. doi: 10.1016/j.bone.2013.01.020

Figure 5. Kinetic analysis of polyphosphate hydrolysis catalyzed by recombinant TNAP.

Figure 5

Kinetic activity of TNAP on (A) PolyP5 and (B) PolyP65 was measured based on released Pi as described in Material and Methods. Shown are the reaction velocities (activity) as a function of the log of substrate concentration. Insets show kinetic analysis in the presence of 0.5 mM of MgCl2 for (A) PolyP5 and (B) PolyP65. Data are presented as means ± SE.