Fig. 7.

Effects of Raf-1 kinase inhibition on PKC-induced Raf-1 (a), MLC₂₀ (b), MYPT1 (c), MEK (d) and ERK (e) phosphorylation in rat VSMCs. Cultured cells were pretreated with vehicle or GW5074 (10 μM) or U0126 (10 μM) or calphostin C (100 nM) for 30 min before stimulated with 3 μM PDBu for 15 min. Treated cells were processed as described in Methods. The phosphorylation levels of these proteins were determined by immunoblotting. Top panels show representative blots of the respective phospho-protein and β-actin, bottom panels are the summary of densitometric results. Results were normalized against β-actin and expressed as folds change relative to untreated control. Data represent means of 3 independent experiments. * p < 0.05 compared to vehicle-treated control.