. Author manuscript; available in PMC: 2013 Jul 16.

Published in final edited form as: Arq Bras Endocrinol Metabol. 2012 Dec;56(9):646–652. doi: 10.1590/s0004-27302012000900008

Table 2.

Oligonucleotide sequences and annealing temperature (°C) of TAC3 and TACR3 genes PCR.

Gene	Exon	Oligonucleotide Sequence	Annealing Temp (°C)
*TACR3*	1	1F 5′CAGGGATTGCAGTATCTTTC 3′ 1R 5′CCTCCTTTCAGCAAAAATTC3′	55
	2	2F 5′GCCATGATTACCATTCTACG3′ 2R 5′ACTTATTGACCACACACAAATC3′	56
	3	3F 5′CAACTGGCAGCATTTGAAAC3′ 3R 5′GATTACAGTATGTGGACAGCAGC3′	56
	4	4F 5′CTGTCCGTATATTGCTTCACC3′ 4R 5′AAGCCTGTGCCTCTCTCAG3′	55
	5	5F 5′TGACATAAATTCTAAGAGTCTGG3′ 5R 5′CTTTCTCAATTTGACCATAGC3′	55
*TAC3*	2	2F 5′CAAGCTGCTGGTAATGAATG3′ 2R 5′AAATGCCCTCTGACGGAC3′	55
	3 and 4	34F 5′GATTCAGGATGGGCTCAGG3′ 34R 5′GGGAGCTGGCATATTGTTTG3′	55
	5 and 6	56F 5′GAACAGAGACCAGAAACCCAGTC3′ 56R 5′TTTAATACCTGTAGCATGGGAGGAA3′	56

F: forward R: reverse