Fig. 7.
Shh concentration affects expression of α-actin in fetal mouse bladders: (A) Western blot analysis of whole mouse fetal bladders after 72 h of incubation in serum free DMEM and increasing concentrations of Shh. (B) Expression of smooth muscle α-actin in intact bladders relative to β-actin incubated with various concentration of Shh after 72 h. There was no significant difference in the level of α-actin expression between bladders cultured in 480 nM of Shh and those cultured in 48 nM of Shh. SMAA=smooth muscle α-actin, β-actin=control.