Table 2. Sequences, GC content and reads for the top 50 small RNAs from four pART27-42 hairy root lines.

SmallRNAID	small RNA sequence	Sequencelength	GC%	No. of GuidessRNA	No. of PassengerssRNA	Guide/passengerRatio
16D10	GGCAAAAAGCCUAGUGGGCCAAAUCCUGGAGGAAAUAAUUGA	42	45.2
R1	UAGUGGGCCAAAUCCUGGAGG	21	57.1	643	78930	0.01
R2	CCUAGUGGGCCAAAUCCUGGA	21	57.1	9745	800	12.18
R3	CUAGUGGGCCAAAUCCUGGAG	21	57.1	1393	6340	0.22
R4	AGCCUAGUGGGCCAAAUCCUG	21	57.1	6704	703	9.54
R5	AAAGCCUAGUGGGCCAAAUCC	21	52.4	6414	6	1069
R6	AAUCCUGGAGGAAAUAAUUGA	21	33.3	1649	4657	0.35
R7	GUGGGCCAAAUCCUGGAGGAA	21	57.1	5603	90	62.26
R8	UGGGCCAAAUCCUGGAGGAAA	21	52.4	5100	216	23.61
R9	AAGCCUAGUGGGCCAAAUCCU	21	52.4	3981	70	56.87
R10	GGCCAAAUCCUGGAGGAAAUA	21	47.6	3375	118	28.6
R11	AGUGGGCCAAAUCCUGGAGG	20	60	75	3197	0.02
R12	AAAUCCUGGAGGAAAUAAUUG	21	33.3	136	2439	0.06
R13	GCCAAAUCCUGGAGGAAAUAA	21	42.9	2231	149	14.97
R14	GGGCCAAAUCCUGGAGGAAAUAAU	24	45.8	2308	21	109.9
R15	UGGGCCAAAUCCUGGAGGAAAUAA	24	45.8	1978	16	123.63
R16	AAUCCUGGAGGAAAUAAUUG	20	35	99	1693	0.06
R17	AAAGCCUAGUGGGCCAAAUCCUGG	24	54.2	38	1719	0.02
R18	GCCUAGUGGGCCAAAUCCUGG	21	61.9	383	1318	0.29
R19	UCCUGGAGGAAAUAAUUGAGG 1	21	42.9	0	1696	0
R20	CCUAGUGGGCCAAAUCCU	18	55.6	224	1336	0.17
R21	CCUAGUGGGCCAAAUCC	17	58.8	222	1110	0.2
R22	GUGGGCCAAAUCCUGGAGGAAAUA	24	50	415	722	0.57
R23	UAGUGGGCCAAAUCCUGGAG	20	55	116	1012	0.11
R24	GGCCAAAUCCUGGAGGAAAUAAUU	24	41.2	744	327	2.28
R25	AGUGGGCCAAAUCCUGGAGGA	21	57.1	740	286	2.59
R26	UAGUGGGCCAAAUCCUGGAGGAAA	24	50	815	208	3.92
R27	AAAUCCUGGAGGAAAUAAUUGAGG 1	24	37.5	0	967	0
R28	GGGCCAAAUCCUGGAGG	17	64.7	9	906	0.01
R29	UAGUGGGCCAAAUCCUGG	18	55.6	38	699	0.05
R30	AAAUCCUGGAGGAAAUAAUU	20	30	68	623	0.11
R31	CAAAUCCUGGAGGAAAUAAUU	21	33.3	564	117	4.82
R32	GGGCCAAAUCCUGGAGGAAAUAA	23	47.8	653	25	26.12
R33	GCCAAAUCCUGGAGG	15	60	26	647	0.04
R34	CCUAGUGGGCCAAAUCCUGG	20	60	435	237	1.84
R35	CUAGUGGGCCAAAUCCU	17	52.9	306	356	0.86
R36	GGCCAAAUCCUGGAGG	16	62.5	22	630	0.03
R37	AGUGGGCCAAAUCCUGG	17	58.8	25	551	0.05
R38	GUGGGCCAAAUCCUGG	16	62.5	510	62	8.23
R39	CUAGUGGGCCAAAUCCUGGA	20	55	322	240	1.34
R40	AAGCCUAGUGGGCCAAAUCCUG	22	54.5	485	44	11.02
R41	UAGUGGGCCAAAUCCU	16	50	43	484	0.09
R42	UGGGCCAAAUCCUGGAGGAA	20	55	491	32	15.34
R43	GGGCCAAAUCCUGGAGGAAAU	21	52.4	454	56	8.11
R44	AGCCUAGUGGGCCAAAUCCU	20	55	476	37	12.86
R45	AAUCCUGGAGGAAAUAAUU	19	31.6	39	466	0.08
R46	CUAGUGGGCCAAAUCCUGGAGG	22	59.1	38	457	0.08
R47	GCCAAAUCCUGGAGGAAAUA	20	45	301	166	1.81
R48	AAAAAGCCUAGUGGGCCAAAUCCU	24	45.8	429	10	42.9
R49	CCUAGUGGGCCAAAUCCUG	19	57.9	364	64	5.69
R50	UAGUGGGCCAAAUCCUG	17	52.9	120	307	0.39

¹Nucleotides in italic were derived from the loop sequence of pART27-42.