Table 1. RNA-Seq statistics of E2, P4, TAM, and RU486 treated and non-treated Ishikawa cells.
Ishikawa E2 & TAM | Ishikawa P4 & RU486 | ||||||
Non-treated | 3 h E2 | 12 h E2 | 12 h TAM | 3 h P4 | 12 h P4 | 12 h RU486 | |
Reads aligned to Tophat | 10 465 431 | 9 967 453 | 8 093 476 | 10 912 784 | 10 628 213 | 11 058 071 | 10 983 639 |
Total genes | 16813 | 16874 | 16784 | 16834 | 16951 | 16934 | 16840 |
% unknown genes | 29.07% | 29.09% | 28.89% | 28.86% | 29.43% | 29.24% | 29.06% |
% known genes | 70.93% | 70.91% | 71.11% | 71.14% | 70.57% | 70.76% | 70.94% |
FPKM 0–10 | 4577 | 4361 | 4226 | 4611 | 4437 | 4406 | 4607 |
FPKM 10–100 | 6487 | 6701 | 6894 | 6486 | 6653 | 6722 | 6495 |
FPKM 100–1000 | 820 | 859 | 767 | 831 | 831 | 807 | 804 |
FPKM >1000 | 41 | 44 | 48 | 47 | 41 | 47 | 40 |
Significant genes * | 1084 | 1121 | 1013 | 1082 | 1097 | 546 | |
% endometrium (n = 2) ** | 98.6% | 99.2% | 98.6% | 98.2% | 99.0% | 98.5% |
Significant genes (5% FDR) are counted from known genes and compared to non-treated cells.
E2 and P4 significant genes present in human endometrium during the time of embryo implantation.