Skip to main content
. 2013 Jul 16;8(7):e69338. doi: 10.1371/journal.pone.0069338

Figure 5. The expression of metabolic genes in the liver of dams fed different proportions (10/73, 20/63 or 30/53%) of dietary protein/dietary carbohydrates (DP/DCH) during gestation and lactation.

Figure 5

(A) The relative mRNA levels of sterol regulatory element binding protein 1 (SREBP1), fatty acid synthase (FAS), and pyruvate kinase (PK). (B) A representative immunoblot of AMP-activated protein kinase (AMPK), threonine phosphorylation of AMP-activated protein kinase (P-AMPK), p70 S6 kinase (S6K), threonine phosphorylation of S6 kinase (P-S6K), fatty acid synthase (FAS) and actin. (C) Western blot densitometric analysis of FAS/ACTIN. (D) A representative histology picture of the liver of rats fed 10/73, 20/63 or 30/53% DP/DCH at day 5 of lactation. Western blot densitometric analysis of (E) P-S6K/S6K, (F) P-AMPK/AMPK. (G) The relative mRNA levels of serine dehydratase (SDH). Values are the mean ± SEM of three different blots. n = 5. **p<0.01, ***p<0.001.