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. 2013 Jul 16;8(7):e69161. doi: 10.1371/journal.pone.0069161

Figure 4. Sc-SRP-6 inhibits chymotrypsin-like enzymes by a classical mechanism involving RCL.

Figure 4

A–B: Lineweaver-Burk double reciprocal plots showing that Sc-SRP-6 is a competitive inhibitor of trypsin and α-chymotrypsin. C – D: Formation of SDS-stable complexes of Sc-SRP-6/trypsin and Sc-SRP-6/α-chymotrypsin. An increased SI for Sc-SRP-6 was used (5, 10 and 15 in wells 1, 2 and 3). A serpin/protease complex (asterisk), a serpin (black arrow), a hydrolysed serpin (white arrows) and a degraded serpin (grey arrow) are indicated at specific sites in each gel.