Figure 5. Sc-SRP-6 inhibits insect midgut enzymes.

A: Inhibitory activity of Sc-SRP-6 against trypsin and chymotrypsin in insect digestive juices. The juices were partially fractionated by gel filtration, and the inhibitory activity tested using BApNA and AAPPpNA substrates for trypsin and chymotrypsin. B: P. unipuncta larvae fed a diet incorporated with albumin-bromphenol blue show faeces with no traces of colour (control), and larvae fed on the same diet supplemented with 0.2% Sc-SRP-6 (w/v) retained the coloured compound. C: Incremental weight of larvae treated with 0.2% Sc-SRP-6 (w/v) compared with untreated controls. The bars represent standard deviations from three independent replicates. The different letters indicate significant differences (p<0.05).