Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2013 Jul 16;8(7):e68154. doi: 10.1371/journal.pone.0068154

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

PMC Copyright notice

I. Western blot (using anti-MMP-2 IgG) of ventricular mitochondrial lysates isolated from 2–3 progeny of three individual transgenic founder lines (3393, 3930 and 3941). The NTT-MMP-2/EGFP fusion protein has an apparent molecular mass of 92 kDa and there is evidence for proteolytic cleavage of the EGFP component in several of the hearts, leaving the 65 kDa NTT-MMP-2 protein intact (+C: recombinant NTT-MMP-2/EGFP fusion protein). II. Immunohistochemistry of wild type (WT, panel A) and transgenic (TG, panels B–C) ventricular sections probed with anti-EGFP antibody and examined using Nomarksi optics. Compared to the WT controls, immunostaining is present in dense clusters (green pseudocolor) extending longitudinally between the myofilaments. In addition, linear arrays of reaction product are present in a subsarcolemmal distribution (panel C, arrows) as well as perpendicularly across the long axis of individual cardiomyocytes (panel D, arrows). The staining distribution is consistent primarily with a mitochondrial localization of the NTT-MMP/EGFP protein. (Final magnification: A, B: X325; C: X600; D: X900).