Table 2. Cross-recognition results for six different Melan-A-specic CTL clones in a chromium release assay: the logarithm of the relative functional avidity of the ELX analogs to ELA is given.
Seqa | Melan-A-specic CTL clones | |||||||||||
203-R7 | 25-R3 | 203-R2b | 203-R1 | 203-R3 | 25-R35 | |||||||
ELA | 0.00c | 0.00d | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 |
ELS | −0.59 | 0.14 | −0.41 | 0.13 | −0.07 | 0.00 | −0.01 | 0.10 | −0.99 | 0.01 | −2.44 | 0.50 |
ELT | −0.19 | 0.32 | −2.17 | 0.11 | 0.09 | 0.00 | −0.05 | 0.15 | −1.88 | 0.08 | −1.59 | 0.62 |
The P1-P3 peptide sequence of the ELX analog.
Only one experiment was done with the 203-R2 clone.
The logarithm of the relative functional avidity of the ELX analogs compared to ELA was calculated as log10(EC50ELA/EC50ELX). A value of −1.00 means that the molar concentration of the ELX peptide needs to be 10 times higher than ELA to achieve the same activity, i.e. 50% of maximal lysis. The average over the independent experiments for each CTL clone is given.
Standard deviation. By the denition of the score, the standard deviation for ELA is zero.