. 2013 Jan 9;143(3):315–323. doi: 10.3945/jn.112.171322

TABLE 2.

Hepatic fatty acid composition in Ldlr^−/− mice fed the NP, WD + OO, WD + EPA, WD + DHA, and WD + EPA + DHA diets for 16 wk¹

Fatty acid	NP	WD + OO	WD + EPA	WD + DHA	WD + EPA + DHA
			μmol/g protein
14:0	5 ± 2^c	92 ± 11^a	57 ± 11^b	52 ± 12^b	56 ± 9^b
16:0	339 ± 77^c	1970 ± 284^a	1720 ± 320^a,b	1360 ± 327^b	1490 ± 206^b
16:1 (n-7)	30 ± 13^c	461 ± 57^a	445 ± 81^a	293 ± 82^b	363 ± 86^a,b
18:0	141 ± 11^b	178 ± 25^a	178 ± 18^a	153 ± 23^a,b	158 ± 14^a,b
18:1 (n-9)	196 ± 65^d	4140 ± 591^a	2930 ± 599^b	2030 ± 610^c	2460 ± 457^b,c
18:1 (n-7)	39 ± 9^d	847 ± 127^a	411 ± 104^b	204 ± 87^c	279 ± 103^b,c
18:2 (n-6)	320 ± 107^a,b	355 ± 46^a,b	413 ± 76^a	278 ± 44^b	333 ± 52^a,b
18:3 (n-3)	10 ± 5^b,c	9 ± 2^c	16 ± 4^a	11 ± 3^a,b,c	15 ± 4^a,b
20:0	4 ± 1^c	134 ± 20^a	74 ± 16^b	53 ± 22^b	62 ± 13^b
20:2 (n-6)	2.6 ± 0.3^c	8.1 ± 1.0^a	7.4 ± 1.7^a	2.3 ± 0.7^c	5.2 ± 0.9^b
20:3 (n-6)	8 ± 1^c	22 ± 3^a	24 ± 3^a	9 ± 1^c	16 ± 2^b
20:4 (n-6)	76 ± 8^b	142 ± 20^a	67 ± 8^b	31 ± 5^c	39 ± 5^c
20:5 (n-3)	8 ± 2^c	2 ± 1^c	115 ± 32^a	65 ± 8^b	92 ± 18^a
22:5 (n-6)	2 ± 1^b	26 ± 8^a	8 ± 1^b	6 ± 3^b	6 ± 3^b
22:5 (n-3)	7 ± 1^d	7 ± 2^d	126 ± 27^a	47 ± 13^c	77 ± 13^b
22:6 (n-3)	63 ± 11^c	55 ± 8^c	121 ± 21^c	424 ± 97^a	313 ± 84^b
Sum C20-22²
C20-22 (n-3)	78 ± 14^c	64 ± 9^c	362 ± 78^b	536 ± 113^a	482 ± 109^a
C20-22 (n-6)	91 ± 8^b,c	211 ± 30^a	108 ± 13^b	51 ± 7^d	68 ± 7^c,d
Fatty acid ratios³
20:4 (n-6):18:2 (n-6)	0.26 ± 0.02^b	0.40 ± 0.04^a	0.16 ± 0.02^c	0.11 ± 0.02^c	0.12 ± 0.02^c
20:3 (n-6):18:2 (n-6)	0.03 ± 0.003^c	0.06 ± 0.007^a	0.06 ± 0.007^a,b	0.03 ± 0.005^c	0.05 ± 0.004^b

Values are mean ± SD, = 8/treatment group. Labeled means in a row with superscripts without a common letter differ, P ≤ 0.05. NP, nonpurified diet; WD + DHA, Western diet supplemented with DHA; WD + EPA, Western diet supplemented with EPA; WD + EPA + DHA, Western diet supplemented with EPA and DHA; WD + OO, Western diet supplemented with olive oil.

Sum C20-22 refers to the sum of all the 20 to 22 carbon fatty acids analyzed.

Fatty acid ratios refers to the mole ratio of 20:4 (n-6):18:2 (n-6) or 20:3 (n-6):18:3 (n-6).