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. 2013 Jul 16;8(7):e68441. doi: 10.1371/journal.pone.0068441

Figure 1. Targeted deletion of FgLaeA from the genome of the F. graminearum Z3643 (WT), FLTRI6, or FLZEB2 strains.

Figure 1

(A) Deletion scheme. (B) BglII-digested genomic DNA gel blot hybridized with probe 1 (upper panel) and probe 2 (lower), respectively; positions are indicated in A. Lanes 1, 2, and 6: the Z3643, FLTRI6, and FLZEB2 strains; lanes 3 and 4: the FgLaeA-deletion strains derived from Z3643 (designated ΔFgLaeA); lanes 5 and 7: the FgLaeA-deletion strains derived from FLTRI6 (FLTRI6ΔFgLaeA); lane 7: the FLZEB2ΔFgLaeA strain; lanes 9 and 10: the FgLaeA add-back strains derived from the ΔFgLaeA strain; lane 11: the FgLaeA-overexpression strain derived from the ΔFgLaeA strain. DNA size markers are indicated on the left side of the gel. Note that a ∼7.5-kb nonspecific hybridizing band was observed in all of the strains examined when hybridized with probe 1.