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. 2013 Jul 16;8(7):e68548. doi: 10.1371/journal.pone.0068548

Figure 5. Mef2C/MyoD sites are essential for SME8/16 muscle specific expression.

Figure 5

Tol2 constructs containing tandem kidney (KE) and muscle (SME) enhancers in front of γ-cry promoter (A) were systematically mutated to remove the predicted Mef2 and MyoD sites (B, H), and compared to the ‘wildtype’ construct in transgenic efficiency and tissue specificity (C, H), as well as expression intensity (G, M). Mutating either Mef2C or MyoD sites reduced the number of embryos expressing in muscle, as well as reduced the expression intensity (D-F; I-K; G, M). [*p-value <0.05; **p-value <0.001].