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. 2013 Jul 16;8(7):e70414. doi: 10.1371/journal.pone.0070414

Figure 4. Heart physiology at 1 min and 1 h following FlyNap anesthesia.

Figure 4

Mosquitoes were anesthetized by exposure to FlyNap (FN) for 30 sec, and heart recordings were acquired at 1 min and 1 h following FlyNap treatment. The following heart parameters are reported: (A–C) total, anterograde and retrograde contraction rate; (D) frequency of heartbeat directional reversals; (E–F) percent time contracting in the anterograde and retrograde directions; (G–H) anterograde and retrograde period lengths. Data were analyzed using a paired t-test, and the statistical significances between the groups are denoted with an asterisk (ns, P>0.05; *, P=0.0484). The percentages reported in each graph report the percentage change between 1 min and 1 h post-FlyNap treatment. Bars denote the standard error of the mean. N = 10.