Figure 1. ATP depletion and viability comparison of supercooled rat hepatocytes with other preservation modalities.

A) Comparison of ATP retention of cells at different temperatures in HTS solution; cells preserved at -4 and -7 ^oC retain statistically similar amounts of ATP (p>0.05) while ATP retention at both temperatures are significantly higher compare to -10 and +4^oC on both days B) Comparison of ATP retention for cells preserved in HTS and UW solutions at +4 and -4 ^oC. ATP retention among the two solutions are statistically similar (p>>0.05) at either -4 or 4 ^oC; however, there is a a statistically significant (p<0.05) difference in ATP retention between -4 and at 4⁰C at and beyond 3 days of preservation. C) Cell viability after 1 hour of suspension rewarming monitored up to 7 days of preservation; any two values that do not have overlapping error bars on this graph is significantly different from each other (p<0.05).