Figure 2. Agglutination ability of heterothallic strains harboring deletion of the pheromone receptor gene.

The strains used were L972 (h ^– wild type), L975 (h ⁺ wild type), FS65 (h ^– mam2Δ), and FS66 (h ⁺ map3Δ). Cells were incubated with gentle shaking in SSL−N for 4 hr. AI was determined for triplicate samples. Means with standard deviations (vertical bars) are presented. Visible agglutination was seen in samples with an AI of more than 1.1.