Table 1. Strains used in this study.
Strains | Genotype | Source |
---|---|---|
L968 | h 90 | Leupold U |
L972 | h – | Leupold U |
L975 | h + | Leupold U |
Eg928 | h 90 mam1::ura4 + ura4-D18 | Nielsen O |
FS55a | h 90 mfm1::LEU2 mfm2-D4 mfm3::ura4 + ade6-M210 ura4-D18 leu1-32 | [17] |
FS65 | h – mam2::LEU2 leu1 | This study |
FS66 | h + map3 ::ura4 + ura4-D18 | This study |
FS71 | h90 map4 ::ura4 + ade6-M210 ura4-D18 leu1 | This study |
FS85 | h + ade6-M210 ura4-D18 leu1 | This study |
FS103 | h + map4::ura4 + map4:leu1 + :map4-GFP ade6-M210 | This study |
FS114 | h + ste11::ura4 + ade6-M210 ura4-D18 leu1 | This study |
FS120 | h – mfm1::LEU2 mfm2-D4 mfm3::ura4 + ade6-M210 | This study |
FS121 | h – ade6-M210 | This study |
FS123 | h 90 fus1::kanR ade6-M216 leu1 | This study |
FS127 | h + ura4-D18 | This study |
FS133 | h + ade6-M210 ura4-D18 leu1 [pTA14] | This study |
FS135 | h + ade6-M210 ura4-D18 leu1 [pTA14(map4PRO-lacZ)] | This study |
FS251 | h 90 map 2::ura4 + ade6-M216 ura4-D18 leu1-32 | This study |
FS357 | h – ChrI:o14-kanR | This study |
FS403 | h + ste11::ura4 + ade6-M210 ura4-D18 leu1 [pTA14(map4PRO-lacZ)] | This study |
FS404 | h +S ade6-M210 ChrII:d16-natR | This study |
FS419 | h – mfm1::LEU2 mfm2-D4 mfm3::ura4 + ade6-M216 ura4-D18 leu1-32 ChrI:map3 + -hygR | This study |
FS423 | h – ChrI:map3 + -hygR | This study |
SS1290 | h 90 mfm1::LEU2 mfm2-D4 mfm3::ura4 + ade6-M210 ura4-D18 leu1-32 [pSLF173(map4)] | This study |
SS1463 | h – mam2::LEU2 mam3-mCherry-kanR leu1 | This study |
The S. pombe strains constructed in this study will be deposited in the Yeast Genetic Resource Center of Japan, which is supported by the National BioResource Project YGRC/NBRP. a This strain was obtained from YGRC/NBRP (http:/yeast.lab.nig.ac.jp/nig/).