Figure 9. Double positive thymocytes are reduced in favor of an increased CD4⁺ single positive population in the Snai2 and Snai3 DKO.

FACS analysis was performed to assess T cell populations in the thymus (A), peripheral blood (B), and spleen (C). Cells were assayed for CD4 and CD8 cell surface staining. DP = CD4⁺CD8⁺ double positive cells, CD4 = CD4⁺ single positive, CD8 = CD8⁺ single positive, Results are presented as a percentage of total cells analyzed. One-way ANOVA with Bonferroni post hoc test: * p < 0.05, *** p < 0.001.