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. 2013 Jul 16;8(7):e69216. doi: 10.1371/journal.pone.0069216

Figure 12. Circulating hematopoietic profile of six month old mice.

Figure 12

Retro-orbital bleeds were performed three times for each animal assayed. Three mice were analyzed for WT, Snai2+/- Snai3-/-, and Snai2-/- Snai3+/- genotypes. Only one DKO animal survived to six months of age. FACS was used to assess overall percentages (A) and absolute numbers of each lineage (B) within the peripheral blood. Significance was tested using one-way ANOVA followed by the Bonferroni post hoc test. ** p < 0.01, *** p < 0.001 (C) Snai2-/- Snai3+/- and DKO animals display increased lymphocytes and neutrophils in circulating blood. Cytospins were performed with 30 µl of peripheral blood. Slides were stained with Wright-Giemsa to differentiate between blood cell types. 20x images were photographed and representatives for each genotype are shown.