Mice treated with each of the three antibiotics tested as monotherapy showed similar weight loss to saline control at 72 hours after challenge (Figure 3a, mean +/− SEM, N=10–16). Azithromycin, ciprofloxacin, or tobramycin administration each reduced both wound site (Figure 3b, #P<0.01) and systemic infection of PAO1 (Figures 3c, 3d, ∞P<0.001, #P<0.01) when compared with saline control.
The comparison with saline control was statistically significant and tobramycin appeared to be superior as monotherapy in concentrations tested, but no statistically significant difference was observed between antibiotics. NS, normal saline; AZM, azithromycin; CPRO, ciprofloxacin; TBRA, tobramycin.