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. Author manuscript; available in PMC: 2014 Oct 1.
Published in final edited form as: Transgenic Res. 2013 Mar 15;22(5):965–972. doi: 10.1007/s11248-013-9700-0

Fig. 5. Pancreatic hormone expression in the Nkx2.2flox/flox;Pdx1-cre mutants is similar to Nkx2.2 null mice.

Fig. 5

(a) The relative expression of islet hormones was assessed in the Nkx2.2flox/flox;Pdx1-cre mice and wildtype control littermates at P0. The expression of ghrelin (Ghr), insulin1 (Ins1), insulin2 (Ins2), glucagon (Gcg), pancreatic polypeptide (Ppy) and somatostatin (Sst) appeared similar to the expected pancreas phenotype of the Nkx2.2 null mice. Pdx1-cre mediated recombination of the Nkx2.2flox allele was efficient in reducing Nkx2.2 transcript. E15.5 pancreas tissue from wildtype (b, c) and Nkx2.2flox/flox;Pdx1-cre (d, e) embryos was subject to immunofluorescence for glucagon, ghrelin, insulin and amylase. DAPI marks nuclei. Images are 40X.