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. Author manuscript; available in PMC: 2014 Aug 1.
Published in final edited form as: Cell Microbiol. 2013 Feb 28;15(8):1323–1340. doi: 10.1111/cmi.12117

Figure 6. Active Gc processes are not required to prevent primary granule fusion with Gc phagosomes.

Figure 6

PMNs were exposed to paraformaldehyde (PFA)-fixed Gc for 1 h, and intracellular and extracellular Gc were discriminated from one another along with antibodies directed against CD63 (A) or neutrophil elastase (NE) (B). Extracellular Gc appear red/blue, while intracellular Gc appear blue only, and primary granule proteins appear green. PMNs were also infected with viable Gc; these cells are not depicted but displayed the same staining pattern for neutrophil elastase and CD63 as presented in Figure 2. Arrows indicate phagosomes negative for granule proteins. The percent of CD63-or NE-positive phagosomes containing viable and PFA-fixed Gc is reported in C.