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. Author manuscript; available in PMC: 2014 Aug 1.
Published in final edited form as: J Surg Res. 2013 Mar 14;183(2):869–875. doi: 10.1016/j.jss.2013.02.035

Figure 4.

Figure 4

Alcian blue staining of intestinal grafts was used to assess the integrity of the intestinal mucus layer of the donor intestinal grafts preserved in (A, A′) histidine-tryptophan-ketoglutarate (HTK) or in (B, B′) HTK containing 5% PEG 15–20 (HTK/PEG). The integrity of the mucus barrier in grafts preserved in HTK alone (A, A′) was attenuated relative to grafts preserved in HTK-PEG (B, B′). (C) Alcian blue staining of the mucus barrier was quantified using automated image analysis software.

Blue staining score of donor intestinal grafts preserved in either HTK (■) or HTK containing 5% PEG 15–20 (□). n=10 donor grafts, *p<0.01.