Figure 7. Fungal burden, fungal distribution, and histopathology of brains infected with C. parapsilosis.
WT and gal3−/− mice were infected with 1×108 CFU of C. parapsilosis via tail vein injection. (A) Brain fungal burden from WT and gal3−/− mice at 3, 7, and 21 days post infection. Bars represent mean CFU. Comparisons of fungal burden were made by Wilcoxon rank-sum test. (B) High magnification (×400) of GMS stained brain section of infected gal3−/− mouse 3 days post infection. Black arrow indicates yeast. (C) High magnification (×600) of H&E stain of brain section of gal3−/− mice 3 days post infection. Yellow arrow heads indicate abscesses. Yeast were observed intravascularly, often outlining the contours of capillaries or larger vessels. Yeast were also observed in the parenchyma.