Figure 1.

OGG1 expression in skeletal muscle isolated from KO and Tg animals. A, Skeletal muscle mitochondrial and nuclear fractions were isolated from different genotype littermates of KO, WT, and Tg mice and analyzed by Western blot for the purity of fractions. Lamin A was used as a marker for nuclear proteins, and subunit IV of mitochondrial complex IV and MnSOD were used as markers for mitochondrial proteins. Equal loading was confirmed using Ponceau staining of the membrane. No detected nuclear contamination was present in the skeletal muscle mitochondria and no contamination of mitochondrial proteins was found in nuclear fraction. B, OGG1 activity in mitochondrial and nuclear fractions isolated from skeletal muscle of KO, WT, and Tg mice. Note the almost complete absence of the product in the KO and minimal product in WT animals and increased amount of incised product in mitochondrial fractions isolated from Tg mice.