Figure 7.

Predicted gene targets of identified miRNAs are regulated by E₂. A, Table of predicted miRNA gene targets determined based on algorithm scores of 3 computer prediction programs: Targetscan, microRNA.org, and MicroCosm. B and C, qRT-PCR for sirtuin 1 (SIRT1), glucocorticoid receptor (GR), GABA receptor A1 (GABRA1), and brain-derived neurotrophic factor (BDNF) in the 3-month-old (B), and 18-month-old (C) vHIPP. Results shown are mean fold change ± SEM as compared young vehicle-treated animals. Data were analyzed by one-way ANOVA with treatment as a factor. * indicates statistically significant difference (P < .05).