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Kiss1 gene expression is dramatically reduced in Kiss1Cre/Cre mice. A–D, qRT-PCR of Kiss1 mRNA normalized first to Actb mRNA, then expressed as a percentage of WT levels. E, Immunohistochemistry for kisspeptin (red) in the ARC of the hypothalamus of castrated male WT, Kiss1Cre/Cre and complete Kiss1 KO animals (from left to right). In the center panel, nuclei express GFP driven by the Kiss1 promoter (see Figure 1). Scale bar, 100 μm. *, P < .05; **, P < .005; ***, P < .0005.
