Sexual maturation in Kiss1Cre/Cre mice. PPS (A) and VO (B) were checked daily in WT, Kiss1Cre/+, and Kiss1Cre/Cre males and females, respectively, as markers for circulating gonadal steroids (n = 6-26). Circles represent WT, squares represent Kiss1Cre/+, and triangles represent Kiss1Cre/Cre mice. *, P < .05 by log-rank Mantel-Cox test. Abbreviation: n.s., not statistically significant.