Figure 1.

GnRH neurons express TRPC4α, a PIP₂-regulated isoform. A, Schematic diagram of TRPC4 gene structure and primer locations. The full-length TRPC4 channel, TRPC4α, is identical to TRPC4β except for the additional 251-bp fragment in exon 11. B, Representative gels illustrating the coexpression of GPR54, TRPC4, and TRPC4α mRNAs in selected GnRH neurons. The expected sizes for the PCR products are as follows: for GnRH, 239 bp; for GPR54, 245 bp; for TRPC4, 116 bp; and for TRPC4α, 241 bp. As a negative control, a cell subjected to RT-PCR but without reverse transcriptase (−RT) did not express any of the transcripts. POA tissue RNA was also included as positive control (+, with RT). Abbreviation: MM, molecular markers.