Figure 4.

Effect of Ca²⁺ channel blockers on the kisspeptin activation of TRPC channels. A–D, Representative recordings showing that the kisspeptin (Kp-10)-activated inward current was inhibited by LVA Ca²⁺ channel blocker Ni²⁺ but not by HVA Ca²⁺ channel (L, N, P/Q) blocker amlodipine (AML). V_hold = −65 mV. E, Summary of the effects of Ca²⁺ channel blockers on the kisspeptin-activated inward current. Cells were recorded using a Cs⁺-based internal solution. ***, P < .001 versus vehicle control group (1-way ANOVA)