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. 2013 Jun 6;154(8):2772–2783. doi: 10.1210/en.2013-1180

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Copyright © 2013 by The Endocrine Society

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Figure 5. — Ca²⁺ store depletion does not affect TRPC channel activation by kisspeptin. A–C, Representative recordings showing the kisspeptin (Kp-10)-induced inward current after a GnRH neuron had been exposed to thapsigargin (Tg, 1μM) for 10 minutes (A), internally dialyzed with 30μM myo-IP₃ (IP₃) and 4μM Tg for 13 minutes (B), or internally dialyzed with 2-APB (300μM) for 15 minutes (C). V_hold = −65 mV. D, Summary of the effects of Tg, IP₃, and 2-APB on the kisspeptin-induced inward currents. The control represents kisspeptin response in the presence of vehicle. P > .05 (1-way ANOVA).