Figure 2.

Inducible PAPP-A excision and recombination in various tissues. PCRs of Tam-induced fPAPP-A excision in a 2-month-old male mouse (A), a 4-month-old female mouse (B), and an 8-month-old male mouse, as described in Materials and Methods, are shown. The 288-bp band represents intact fPAPP-A, and the 240-bp band represents the postexcision product. ao, aorta; ht, heart; lv, liver; sp, spleen; ki, kidney; pg, perigonadal fat; sc, subcutaneous fat; me, mesenteric fat; br, brain; ca, calvaria; fe, femur; tb, tibia; qd, quadriceps; so, soleus; sk, skin; th, thymus; go, gonadal tissue.