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Western blot analysis of total STAT3 expression, phosphorylation (p) of leptin signaling proteins STAT3 and ERK1 and -2 in hypothalamic and brainstem tissue of saline-infused (SS), third or fourth ventricle leptin-infused (LS or SL) or double ventricle leptin-infused rats (LL) for 12 days in experiment 1. Data are means ± SEM for 9 rats. Data for a specific parameter that do not share a common superscript are significantly different at P < .05.
