Figure 1.

CART close appositions onto GnRH cells in the preoptic area and AVPV and ARH Kiss1 cells. Coexpressing CART/α-MSH-ir (cyan) fibers were found in close apposition (white arrows) to preoptic area GnRH-GFP cells (A, pseudocolored red) as well as AVPV Kiss1-ir cells (B, red) and ARH Kiss1-ir cells (C, red). Corresponding brain regions where photomicrographs were taken are illustrated. C₁–C₃, single optical slice analysis at 1-μM focal planes illustrating the close apposition of the CART fiber to the ARH Kiss1 cell shown in (C). 10 μM scale bars are given.