Figure 4.

Identification of ARA metabolites in Dami cells. The HPLC chromatogram shows the 15 identified ARA metabolites in Dami cells. The cells were incubated with 100 μM ARA for 12 h. ARA metabolites were then extracted by ethyl acetate (pH 3−4). Ethyl acetate was evaporated, and the residue was dissolved in 100 μl ethanol, followed by LC-MS/MS screening for ARA metabolites. Further details are included in the “Materials and Methods” section. 20-HETE-6 (100 μg/ml) was used as internal standard.