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. 2013 May 31;49(7):492–500. doi: 10.1007/s11626-013-9633-1

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© The Author(s) 2013

Open Access This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.

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Figure 5. — The effect of 3-MC on ARA metabolism in Dami cells. Dami cells were treated with 10 μM 3-MC for 72 h. The control group of Dami cells received the same final volume of the solvent (DMSO) without 3-MC. After 3-MC treatment, 3-MC-treated Dami cells were divided into two groups. One group of Dami cells (1 × 10⁶ cells) was incubated with 100 μM ARA for 12 h. The other group received 40 μM of SKF-525A in addition to ARA. ARA metabolites, 14,15-EET (A), 14,15-DHET (B), and 20-HETE (C) were identified by LC-MS/MS after liquid/liquid extraction by ethyl acetate. Further details are described in the “Materials and Methods” section. The internal standard utilized was 20-HETE-6 (100 μg/ml). Statistically significant differences as compared with the DMSO treatment group are indicated as *P < 0.05, **P < 0.01, and ***P < 0.001, based on two-tailed Student’s t tests. Data are presented as the mean ± S.D. of reactions performed in triplicate.