Fig. 2.

Induction of an ectopic kinetochore causes genomic rearrangements. 3T3-lacO cells expressing GFP-LacI or GFP-CENP-T-ΔC-LacI were grown in the absence of IPTG for 3 days and then prepared for FISH analysis using probes against chromosome 3 (pink) and LacO sequence (green). Images show representative metaphase spreads after FISH. Note that nonspecific background signal from chromosome 3 probes is also visible on centromeres. Scale bar shows 10 μm. Insets show examples of chromosome rearrangements. Graph shows quantification of the number of chromosomes containing chromosome 3 sequences. N > 15 cells per treatment