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. 2012 Nov 28;32(48):17502–17513. doi: 10.1523/JNEUROSCI.3561-12.2012

Figure 8.

Figure 8.

Intracellular APs from naive, injured (3–5 d postaxotomy), scrambled siRNA-treated, and Kv9.1 siRNA-treated neurons and the effect of elevated [K+]e on excitability. A, Left, Representative APs evoked by dorsal root stimulation from a naive (gray) or an injured (black) cell. Arrow indicates the stimulus artifact. Right, Responses to a series of depolarizing current injections from a naive and an injured neuron at 3, 6, and 9 mm [K+]e. B, Left, Representative APs from scrambled- and Kv9.1 siRNA-treated neurons. The inset highlights the shortened AHP in Kv9.1 siRNA-treated neuron on a different scale. Right, neuronal responses to a series of depolarizing current injections from control or Kv9.1 siRNA-treated neurons at 3, 6, and 9 mm [K+]e. C, Comparison of frequency distribution of threshold current at elevated [K+]e (6–9 mm) between naive/injured neurons (left) and scrambled/Kv9.1 siRNA-treated neurons (right). Dashed lines indicate the respective cumulative percentages, illustrating the shift toward lower values in injured and Kv9.1 siRNA-treated neurons. D, representative AP from a naive neuron in the absence (black) or presence (gray) of the Kv2 blocker stromatoxin-1 (ScTx). The inset highlights the shortened AHP caused by ScTx, similar to Kv9.1 siRNA treatment.