Figure 3.

The hac1 mutants are resistant to the inhibitory effects of high concentrations of exogenous sugars on early seedling development. Mutant and wild-type Col-0 (WT) seeds were surface sterilized and then incubated in the dark at 4°C for 3 days prior to being sown on solid Arabidopsis minimal media supplemented with the indicated additive. After an additional 14 days in the light at room temperature, seeds were scored to determine the percentages of seeds that had germinated, formed expanded cotyledons and developed true leaves. Error bars indicate standard deviations. Mutant and wild-type results differed with: ^*p < 0.05; or ^**p < 0.01, according to a Student's t-test. N = 4.