Figure 4.

The hac1 mutants are moderately resistant to the inhibitory effects of paclobutrazol on seed germination. Mutant and wild-type Col-0 (WT) seeds were surface sterilized, imbibed in the dark at 4°C for 3 days and then sown on Petri plates with Arabidopsis minimal media and 0 or 34 μM paclobutrazol. The Petri plates were incubated under continuous light at 22°C and scored for seed germination at regular intervals. The spy-3 mutant (Jacobsen and Olszewski, 1993) was included as a positive control. Error bars indicate standard deviations. N = 2.