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. 2013 Jul 1;27(13):1473–1483. doi: 10.1101/gad.209619.112

Figure 4.

Figure 4.

SIRT1 is a downstream target of miR-138 in adult sensory neurons during axon regeneration. (A) R-luc activity assay in CAD cells coexpressing the luciferase reporter containing the full-length SIRT1 3′ UTR and the miR-138 mimics or inhibitor. Note that expression of miR-138 mimics inhibited, while expression of the miR-138 inhibitor enhanced, the luciferase activity. n = 3; (**) P < 0.01. (B) Mutation of the miR-138 targeting site in the SIRT1 3′ UTR abolished the regulation of luciferase activity by miR-138 mimics or its inhibitor. n = 3. (C) Overexpression of the miR-138 mimics in cultured adult DRG neurons led to decreased endogenous SIRT1 protein level. (D) Electroporation of the miR-138 mimics into adult DRGs in vivo led to decreased endogenous SIRT1 protein level.