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. 2013 Jul 1;27(13):1473–1483. doi: 10.1101/gad.209619.112

Figure 5.

Figure 5.

SIRT1 regulates sensory axon regeneration in vivo. (A,B) Both SIRT1 mRNA (n = 9; [**] P < 0.01) and protein levels were increased in adult DRGs 1 wk after sciatic nerve lesion compared with the naïve uninjured DRGs. (C) In vivo electroporation of SIRT1 siRNA oligos (siSIRT1) markedly knocked down SIRT1 protein level in DRGs after 3 d. (D) Average lengths of regenerating sciatic nerve axons. n = 7 mice for the control group; n = 15 mice for the SIRT1 siRNA group: (***) P < 0.001. (E) Cumulative distribution of the lengths of all individual axons measured. n = 249 for control; n = 545 for SIRT1 siRNA. (F) Representative images of EGFP-labeled regenerating axons in the whole-mount sciatic nerves. The crush sites were marked by the epineural suture (red lines). Bar, 1 mm.