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. 2013 Jul 1;27(13):1473–1483. doi: 10.1101/gad.209619.112

Figure 6.

Figure 6.

SIRT1 represses miR-138 transcription in adult DRG neurons. (A) Knocking down SIRT1 with siRNA (siSIRT1) in cultured adult DRG neurons led to increased expression of miR-138 in vitro. n = 7; (**) P < 0.01. (B) Knocking down SIRT1 in adult DRGs in vivo led to increased expression of miR-138 in vivo. n = 4; (*) P < 0.05. (C, top) Schematic drawing of the 4-kb genomic regions (R1–R5) proximal to the miR-138-1 gene on chromosome 8 that were assayed in the ChIP experiment. (Bottom) In the SIRT1 ChIP experiment, the genomic region R3 was amplified from injured adult DRG tissues, but not the naive tissues, to bind to SIRT1. (D) RT-PCR data quantifying the enrichment of the binding between SIRT1 and the R3 genomic region of the miR-138-1 locus in adult DRG tissues. n = 4; (*) P < 0.05. Note that SIRT1 only binds to the R3 region in response to the peripheral axotomy.