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. 2013 Jul 1;27(13):1473–1483. doi: 10.1101/gad.209619.112

Figure 7.

Figure 7.

SIRT1 is both the input and output signals of the miR-138/SIRT1 regulatory loop to control axon regeneration. (A) qRT-PCR data indicating endogenous SIRT1 mRNA levels in adult DRGs after peripheral nerve injury. Note that SIRT1 expression was significantly up-regulated at 0.5 d after peripheral axotomy compared with the uninjured naïve DRGs. n = 8 for each time point; (*) P < 0.05; (**) P < 0.01. (B) qRT-PCR data indicating endogenous miR-138 levels in adult DRGs after peripheral nerve injury. Note that miR-138 expression was significantly down-regulated at 1 d after peripheral axotomy compared with the uninjured naïve DRGs. n = 8 for each time point; (*) P < 0.05; (**) P < 0.01. (C) Overexpression of miR-138 and inhibition of SIRT1 activity with the inhibitor EX527 had no additive inhibitory effects on regenerative axon growth from adult DRG neurons. n = 3; (**) P < 0.01. (D) Expression of SIRT1 lacking the 3′ UTR, which cannot be targeted by miR-138, was able to fully rescue axon growth of adult DRG inhibited by miR-138 expression. n = 3; (*) P < 0.05. (E) Inhibition of miR-138 with the inhibitor (anti-138) only partially rescued regenerative axon growth inhibited by knockdown of SIRT1 expression. n = 7; (**) P < 0.01.