Table 3.
Impact of Alamethicin and Saccharolactone on HLM150 Specific Activity Toward R- and S-warfarin used in Correlative Studiesa
| Substrate | Reaction Adjunct | 6-Hydroxywarfarin | 7-Hydroxywarfarin | 8-Hydroxywarfarin | 10-Hydroxywarfarin | 4′-Hydroxywarfarin |
|---|---|---|---|---|---|---|
| R-warfarin | absence presence |
0.0362 (0.0017) 0.0342 (0.0022) |
0.0389 (0.0019) 0.0391 (0.0021) |
0.0105 (0.0023) 0.0095 (0.0013) |
0.113 (0.003) 0.121 (0.007) |
0.0444 (0.032) 0.0382 (0.027) |
| S-warfarin | absence presence |
0.183 (0.005) 0.179 (0.006) |
0.770 (0.018) 0.785 (0.015) |
0.269 (0.014) 0.271 (0.012) |
0.226 (0.010) 0.216 (0.013) |
0.0745 (0.0027) 0.0727 (0.0022) |
a
Units for observed rates are nmol/min/mg protein. Standard deviation for average of values are shown in parentheses.